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You are not working in a still air box, as you’re processing far too many vials to not have a fully sterile environment."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"You have stoppers in a sterile container that you can open in front of the laminar flow so they will stay sterile."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"You have multiple wrapped sterile tweezers that you will use to pick up the stoppers. If you accidentally drop one or touch it to something non-sterile you can swap it out for one of your sterile, unused ones."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"All of your vials are sterile and still have foil hats on them. They are staged in a way that you have ready access to them as you need them."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"I prefer to dispense with a 20mL syringe with a 18g needle attached. 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I won’t need to dig through any boxes. Everything I need is staged, and anything I won’t need is moved well out of the way."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"My task light is on so I can see clearly."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"I have a helper, Person 2, sitting next to me who understands how this is all about to go down. 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Please don’t skip. Perfect aseptic technique is an essential part of how our vials end up sterile. Make sure your helper understands this as well."]}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"If you work on a riser in front of the flow hood, make sure it’s not positioned so that if something spills it can fall onto the filter."}],"\n",["$","h2",null,{"id":"dispensing","className":"x:tracking-tight x:text-slate-900 x:dark:text-slate-100 x:font-semibold x:target:animate-[fade-in_1.5s] x:mt-10 x:border-b x:pb-1 x:text-3xl nextra-border","children":["Dispensing",["$","$L21",null,{"id":"dispensing"}]]}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":[["$","em",null,{"children":"I’m going to outline a recommended way to work. You may find a workflow that you prefer more"}],"."]}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":[["$","em",null,{"children":"I’m assuming you’re using 3mL vials and 20mL syringes, adjust as needed"}],"."]}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Person 2 opens the 20mL syringe / 18g needle packaging in the sterile field. They attach the two. They draw up 18mL of air from the sterile field. They use an alcohol swab to clean the stopper of a 100mL vial. The inject 18mL of sterile air, and draw up 18mL of sterile preparation, without large bubbles. They cap the needle. They do this again with another syringe."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"We use 18mL as it is divisible by 3, and we’re using 3mL vials."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Person 1 takes the syringe and in the sterile field removes the cap. They hold the syringe in hand while removing the hat from a sterile vial. Trying not to touch the needle to the side of the vial (difficult for a 3mL vial) they inject 3mL into the vial while still holding it in their hands."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"They set the open vial down near the filter of the flow hood to minimize risk of anything accidentally being set in front of it."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"18mL -> 15mL -> 12mL -> 9mL -> 6mL -> 3mL -> 0mL"}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Person 1 does one vial at a time, lining them up across the sterile field. They are not placed in front of each other, but next to each other. After they’re finished they can set the syringe down, and Person 2 can fill it back up."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Person 1 takes the tweezers and gently places a stopper on each vial that’s been lined up."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Either Person 1 or Person 2 can use their clean, gloved finger to press the stopper into place. Person 2 can place the caps on top of the stoppers and crimp them."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Person 1 continues in the same fashion, filling vials and placing stoppers."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Person 2 continues filling syringes and crimping vials."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"We start with brand new needles and syringes as often as possible with the economics of your setup. Changing them out every time you start on a new 100mL vial would be a decent benchmark, meaning each would get used for dispensing about 3 time."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"Crimping does not have to take place in the sterile field if you have limited room. Once the stopper is pressed down into place the vial is technically sealed. Sometimes pressure in the vial will cause the stopper to pop out of place. If this happens while not in the sterile field you need to throw away the vial."}],"\n",["$","p",null,{"className":"x:not-first:mt-6 x:leading-7","children":"All done!"}]]}]]}]]}]}] a:[["$","meta","0",{"charSet":"utf-8"}],["$","meta","1",{"name":"viewport","content":"width=device-width, initial-scale=1"}]] 7:null 8:null c:[["$","title","0",{"children":"Large Guide: Dispense | HRT Mom"}]]