Filter & Dispense
Preparing
In your work area you have
- Mixed preparation
- 10mL syringes
- 2 27g needles (wrapped)
- a 18g needle (wrapped) (27g okay)
- a sterile 0.22 PTFE syringe filter
- Pre-sterilized and sealed vials (3mL, 5mL, or 10mL)
- Alcohol swabs
- Gloves (on your hands)
Filter & Dispense
- Attach the 18g needle to the syringe
- Draw up from your preparation however much you want to dispense
- Remove the needle
- Attach the filter to syringe
- Attach the 27g needle to the filter
- Alcohol swab the rubber stopper of the sterile vial
- Take the spare 27g needle and pierce the vial, this is a vent to allow air to escape
- Pierce the vial with the syringe/filter/needle
- Push down on the plunger to begin filtration
- Filtration should happen at a slow drip (approx 0.5-2mL per minute)
- Remove both needles when vial is full/filtration finished
If youāre able, leave some air in the syringe when you draw up your preparation. This will make pushing the syringe easier. See the hand filtration tek for more tips.
Verify Filter Integrity
Now we need to make sure the filter didnāt break when you were filtering. A filter breaking is invisible, so the only way to know if it broke is to use the bubble point test.
The test might feel a little complicated but itās really important you do it correctly. Read the linked page to learn and perform this step.
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