HRT Mom is in Beta! You may encounter minor issues with the site content. Stable release expected Summer or Fall 2025 🍁
Skip to Content
Knowledge BaseInstant Pot Sterilization

Instant Pot Sterilization

🌳đŸ©ș

An Instant Pot is good for sterilization in what we call an “Emergency Field Medicine” type situation. This is not an autoclave. I think it’s fair to consider DIY production an “emergency” and so I support its use. Please upgrade to a gravity autoclave if you’re able.

đŸ«•

“Only the Instant Pot brand pressure cooker was able to inactivate G. stearothermophilus endospores, which indicated that it would be the most appropriate choice for a laboratory pressure cooker.”1

How To

Assuming your Instant Pot reaches 115-118°C at 10.2-11.6 psi or greater:

Run your sterilization cycle for at least 150 minutes.

This is true of the IP-DUO80 model2, and a few others they make. They may make other models with different ratings, so check your manual. Greater ratings are encouraged, though lesser ratings are prohibited.

Instant Pot Max Pressure Mode

Some instant pots have a “max pressure” mode, which may or may not get up to 15psi. What’s important to note is that instant pots do not achieve stable pressure or temperature. They phase in and out of various pressures, with their max rated pressure/temperature. You can not use an instant pot at “max” pressure as if it is a standard autoclave. It is not an autoclave.

I highly recommend that if you’re using max pressure mode that you stick with the research that is linked on this page, and still use the instant pot for at least 150 minutes. There is no research that supports a lesser time.

Don’t follow Instant Pot’s Sterilization Program

That program is designed for stuff like canning and cleaning. It was not designed to make injectables with.

Consider Upgrading

An upgrade may be within your price range. Look into stove top autoclaves. They leave less moisture behind and we can be more confident that they sterilized our items.

References

Page based on work from HRT Cat

View the library page for access to some PDFs.

Footnotes

  1. Assessment and verification of commercially available pressure cookers for laboratory sterilization  ↩

  2. Instant Pot DUO manual  ↩

Last updated on