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Knowledge BaseSterilization Temperatures

Sterilization Temperatures

In depth information lives on the pages for the individual methods:

Dry Heat Sterilization Temperatures1

TemperatureTime
170°C/338°F60 mins
160°C/320°F120 mins
150°C/302°F150 mins

Dry Heat Depyrogenation Temperatures

TemperatureTime
250°C/482°F30 mins1
200°C/392°F60 mins2

Moist Heat Autoclave Sterilization Parameters3

Temperature121°C
Pressure15 psi
Time20-60 minutes*

*You’re supposed to choose a time that you can verify is correctly sterilizing your product. Since that’s typically out of scope for DIY operations, you may choose to sterilize for the maximum recommended time. The CDC recommends 30 minutes4.

Instant Pot Sterilization Parameters5

SettingPressure cook high or max
Time150 minutes

References

View the library page for access to some PDFs.

Footnotes

  1. Compounding Sterile Preparations pp 254-255 ↩ ↩2

  2. European Pharmacopoeia Chapter 2.6.8. Pyrogens  ↩

  3. USP 797 section 10.3, 2024 edition ↩

  4. CDC Autoclave Guidelines  ↩

  5. Assessment and verification of commercially available pressure cookers for laboratory sterilization  ↩

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